Inicio Ítems seleccionados Proveedores de datos OAI Créditos Sobre Ayuda

			Búsqueda avanzada
	Proveedor de datos Braz. J. Vet. Res. Anim. Sci. (1) Autor Camargos,Marcelo Fernandes (1) Leite,Rômulo Cerqueira (1) Lessa,Leandro Moreira (1) Reis,Jenner Karlisson Pimenta (1) Rocha,Maurílio Andrade (1) Stancek,Daniel (1) Más... Palabra clave Bovine leukemia virus (1) Gene (1) Immunodiffusion (1) Polymerase chain reaction (1) Tipo del documento Info:eu-repo/semantics/article (1) Año 2003 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevancia Autor Título Año Registros recuperados: 1 Primera ... 1 ... Última Development of a polymerase chain reaction and its comparison with agar gel immunodiffusion test in the detection of bovine leukemia virus infection Braz. J. Vet. Res. Anim. Sci. Camargos,Marcelo Fernandes; Stancek,Daniel; Lessa,Leandro Moreira; Reis,Jenner Karlisson Pimenta; Rocha,Maurílio Andrade; Leite,Rômulo Cerqueira. Polymerase chain reaction (PCR) was used for bovine leukemia virus (BLV) detection in the peripheral leukocytes of the infected bovines. The primers used were designed to amplify a part of env gene of BLV. PCR products were analyzed by agarose gel electrophoresis stained by ethidium bromide. The analytical specificity of PCR was confirmed by enzymatic restriction analysis of the PCR product with Bam HI and also by nucleotide sequence analysis of three PCR samples. Sixty five animals were tested for anti-BLV antibody, by agar gel-immunodiffusion test (AGID) and for direct BLV detection by PCR. There was a 73.80% concordance rate between the two tests. Four animals positive in AGID were PCR negative, while 13 AGID negative animals were found PCR positive.... Tipo: Info:eu-repo/semantics/article Palabras clave: Immunodiffusion; Bovine leukemia virus; Gene; Polymerase chain reaction. Año: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000500005 Registros recuperados: 1 Primera ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária (Embrapa)Empresa Brasileña de Investigación Agropecuaria Todos los derechos reservados, conforme a la Ley 9.610 Política de Privacidad. Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco